Effect of Different Levels and Sources of Supplemental Nano Zinc on Blood-Biochemical Profile and Serum Mineral Status in Wistar Rats (Rattus norvegicus)

This study examined the suitability and comparative efficacy of Zn through different levels and sources on blood-biochemical profile and serum mineral status. 63 weaned (130±3.1g) wistar rats (Rattus norvegicus) divided into 7 equal groups in completely randomized design. These rats were fed a common basal (synthetic) diet for 90 days, except for Zn supplementation, which was 10 ppm through zinc sulphate or 10, 20 and 40 ppm nano Zn either from commercial or synthesized (prepared by green method) source. At the end of the experiment, blood samples were collected from each animal through cardiac puncture and analysed for hemato-biochemistry and serum minerals. The Hb, PCV in blood and glucose, urea, total protein, albumin, globulin and creatinine in serum were comparable (P>0.05) among various treatment groups. Serum Ca, P, Cu, Fe and Mn were also not affected by source and different levels of Zn supplementation, however, serum Zn levels were significantly (P<0.001) higher in nano zinc supplemented groups as compared to control and 10 ppm nano Zn groups. It is concluded that Zn nano particles can be safely supplemented up to 40 ppm level in the diet of rats.