1Division of Veterinary Pharmacology and Toxicology, F.V. Sc. & A.H., SKUAST-J, Jammu and Kashmir, India
2Division of Veterinary Pharmacology and Toxicology, FVAS-IAS, BHU, Varanasi, Uttar Pradesh, India
3Division of Veterinary Anatomy, COVAS, SVPUAT, Meerut, Utter Pradesh, India
*Corresponding author: M Yousuf; Email: yousufdar8@gmail.com
Online published on 7 February, 2019.
The present study was aimed to investigate the effect of aminoguanidine-hemisulphate on amikacin induced oxidative stress in wistar rats of both the sexes. Twenty-four healthy wistar rats were randomly divided into 4 groups. The rats of group-I served as control to which normal saline solution as vehicle was administered inperitoneally once daily for 28 days as sub-acute study period. The rats of group-II and group-III were treated with amikacin (15mg/Kg) and aminoguanidine-hemisulphate (20mg/Kg) daily for 28 days intra-peritoneally, respectively The rats of group-IV were treated with both amikacin and aminoguanidine-hemisulphate at their respective doses and routes once daily for 28 days. The activity of antioxidant stress biomarkers namely SOD, GPx, CAT and GSH levels were significantly decreases on days 15 and 28 after amikacin treatment as compared to control. The oxidative damage was protected in aminoguanidine-hemisulphate treated rats of group-IV as indicated by a significant increase in the activity of SOD, GPx, CAT and GSH-level as compared to amikacin treated rats. The present study suggests that amikacin has the potential of inducing oxidative stress as evidenced by alterations in antioxidant enzymes and GSH. However, Aminoguanidine-hemisulphate has ameliorative effect in normalizing the altered values of various oxidative stress biomarkers.
Amikicin, Aminoguanidine, oxidative biomarkers, wistar rats
