Group A Rotavirus Detection from Diarrhoeal Samples from Buffalo Calves and Human Beings by ELISA and RT-PCR

Rotaviruses belong to family Reoviridae under the genus Rotavirus. It is a major etiological agent of gastroenteritis in buffalo calves. The Mortality due to rotavirus infection in buffalo calves reach up to 25%. Rotaviruses cause neonatal diarrhoea in Buffalo calves. The present study was carried out to determine the prevalence of rotavirus infection among buffalo calves in Anand and adjacent regions. During the present study, 100 diarrhoeal samples were collected from the organized buffalo farms of villages of Anand, Gujarat. Also 55 diarrheic stool samples from the children were collected. All the samples were screened for the presence or absence of rota virus by Double Antibody Sandwich Enzyme linked Immunosorbent Assay (DAS-ELISA) and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) out of the 155 faecal samples, 12 samples (7.74%) were found to be positive for rotavirus by DAS-ELISA and 3 (1.93%) samples were found positive by RT-PCR. None of the human samples were found positive for rotavirus.