1Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, India
2Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, DUVASU, Mathura, India
*Corresponding author: V Pandey; Email: drvijaypandey@gmail.com
Online published on 26 June, 2019.
Adiponectin (ADIPOQ) gene regulates the glucose metabolism, insulin sensitivity, fatty acid oxidation, immunity and reproduction. In the present investigation, PCR-RFLP method was conducted to identify the genetic polymorphism in intron 2 region of adiponectin (ADIPOQ) gene in Indian Sahiwal cattle. PCR of intron 2 of ADIPOQ gene fragment produced amplicon of 961 bp which was subsequently digested with RsaI restriction enzyme. The RsaI/PCR-RFLP assay revealed monomorphic pattern only, TT genotype (wild type homozygote) in Sahiwal cattle population which was confirmed by sequencing. The obtained sequences of ADIPOQ after aligning revealed absence of RsaI recognition site GTAC and consequently, the association study with economic traits could not be performed.
Adiponectin gene, Genetic polymorphism, PCR-RFLP, Sahiwal cattle
