Journal of Animal Research

  • Year: 2019
  • Volume: 9
  • Issue: 4

Microscopic Agglutination Test (MAT) for Leptospirosis in Association with Acute Renal Failure in Dogs

1Veterinary Clinical Complex, F.V. Sc. & A.H., Shuhama (SKUAST Kashmir), Alusteng, Srinagar, Jammu & Kashmir, India

2College of Veterinary and Animal Sciences, GBPUA&T, Pantnagar, Uttarakhand, India

*Corresponding author: NA Tufani; E-mail: tufanivet@gmail.com

Online published on 27 September, 2019.

Abstract

The present investigation was carried out in the Department of Veterinary Medicine, College of Veterinary and Animal Sciences, GBPUA&T, Pantnagar, Uttarakhand, India. Total of 100 sera samples from azotemic dogs were subjected to Microscopic Agglutination Test (MAT) using Leptospira culture in Leptospira lab, Department of Veterinary Bacteriology and Mycology, IVRI, Izatnagar, Bareilly (U.P.) All the sera samples were tested against L. interrogans: Serovars icterohaemorrhagiae, pomona, canicola, autumnalis, grippotyphosa and javanica. Out of 100 sera samples, 20 (20%) were found positive (+) for different serovars of Leptospira interogans and 3 (3%) were doubtful (±) at an antibody titre ≥1: 100. The serovars identified were L. autumnalis (17/100), L. icterohaemorrhagiae (8/100), L. gryppotyphosa (4/100), L. canicola (2/100), L. javanica (2/100) and L. Pomona (1/100). The highest prevalence of Leptospira was observed for serovars L. autumnalis (17%), followed by L. icterohaemorrhagiae (8%) and least for L. pomona (1%).

Keywords

Dog, Leptospirosis, MAT, Prevalence, Renal failure