Journal of Animal Research

  • Year: 2019
  • Volume: 9
  • Issue: 6

Toxigenic Profiling of Enterotoxin-Producing Bacillus cereus Isolated from Marketed Raw Chicken Meat and Human Subjects by Triplex and Multiplex PCR

  • Author:
  • A.P. Suthar1, R. Kumar1,, C.V. Savalia1, N.M. Patel1, I.H. Kalyani2
  • Total Page Count: 9
  • Page Number: 821 to 829

1Department of Veterinary Public Health and Epidemiology, College of Veterinary science & Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India

2Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India

*Corresponding author: R Kumar; E-mail: rajeev37171@gmail.com

Online published on 4 March, 2020.

Abstract

Bacillus cereus incorporates the most important group of endospore-forming micro organism and can cause emetic and diarrheal food poisoning. A total of 42 B. cereus strains isolated from marketed raw chicken meat and human subjects swab samples were assessed by a triplex and multiplex PCR for the presence of enterotoxin genes. The detection rate of nheB, hblA, hblD, cytK, nheA, CER, hblC and entFM enterotoxin genes among all B. cereus strains was 83.33%, 80.95%, 69.04%, 21.42%, 47.61%, 0%, 61.90%, and 92.85% respectively. Enterotoxigenic profiles were determined in enterotoxin-producing strains showed 19 different patterns. The results offer essential information on toxin genes prevalence and toxigenic profiles of B. cereus from sources of origin. The present study was taken into consideration about extreme fitness danger for public health and insuring extra ability in difficulty to food safety amongst all B. cereus group members. Also, there may be need for extensive and continuous tracking of food products embracing both emetic toxin and enterotoxin genes.

Keywords

Bacillus cereus, enterotoxigenic profiling, PCR, enterotoxin, marketed, Chicken, Meat