Department of Plant Protection Faculty of Agriculture, M. H. E. C, University of Tabriz, Tabriz, 516661647, Iran. E-mail: a.mehrvar@tabrizu.ac.ir
1Corresponding Address: Project Directiorate of Biological Control (ICAR), P.B.No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore, 560024, Karnataka, India.
The stability of both crude and semi-purified extracts of different HaNPV isolates under simulated sunlight was studied. It was realized that the persistence of both crude and semi-purified suspensions of Negamum isolate collected from Tamil Nadu was most tolerant to simulated sunlight among all the isolates tested in this study. The highest larval mortality (70.12%) was obtained from crude extract of the Negamum isolate as compared with that of semi-purified suspension (54.44%). The order of inhibition of crude viral extracts of HaNPV isolates under simulated sunlight irradiation at 500 W/m2 for 90 minutes was Negamum≤ Ooty≤ Coimbatore≤ Mumbai ≤ Hyderabad≤ Parbhani≤ Rahuri, which was similar with that of the semi-purified suspensions with the exception of Parbhani ≤ Hyderabad.
Geographic isolates, Helicoverpa armigera, Nucleopolyhedrovirus, persistence, simulated sunlight
