1Centre of Genomics and Bioinformatics, Institute of Agriculture and Natural Sciences, DDU Gorakhpur University, Gorakhpur-273009, Uttar Pradesh, India
2Department of Plant Pathology, Institute of Agriculture & Natural Sciences, DDU Gorakhpur University, Gorakhpur-273009, Uttar Pradesh, India
3Department of Plant Pathology, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya-224229, Uttar Pradesh, India
4Institute of Agriculture and Natural Sciences, DDU Gorakhpur University, Gorakhpur-273009, Uttar Pradesh, India
Radish is an important vegetable crop used in our daily dishes as salad, vegetable and other common cuisines. In the present study, phytoplasma associated with the flat shoot and shoot proliferation son radish plants grown at Narendra Dev University of Agriculture & Technology, Ayodhya campus, India were attempted. The DNA was extracted from diseased and healthy shoots and subjected to nested PCR assays with P1/P7 and R16F2n/R16R2 phytoplasma specific primer pairs. Amplification of 1250 bp product was achieved in all the symptomatic radish samples. The amplified product was cloned and directly sequenced. Sequence analysis of
Candidatus, Diagnosis, Molecular, Phytoplasma, Proliferation, Radish
