Standardisation of high performance liquid chromatographic technique for detection of gentamicin residues from animal tissues

High performance liquid chromatographic (HPLC) technique employing acetonitrile:water as mobile phase, C₁₈ column and fluorescence detector at 260 nm wavelength for excitation and 315 nm wavelength for emission was standardised for detection and quantitation of gentamicin residues. Muscle/organ samples were subjected to solid phase extraction, clean-up procedure and derivatization prior to chromatographic analysis. Gentamicin was eluted in 4 components viz. C₁, C_1a C_2a and C₂ within 15 minutes. Recoveries ranged between 41.89–48.66% in muscle, 45.23–61.21% in liver and 39.80–44.61% in kidney samples fortified with gentamicin at 0.05 to 1.4 ppm levels with detection limit 0.05 ppm.