MicroRNAs (miRNAs) are a large class of small non coding miRNAs that regulate gene expression. In this paper we use the comparative miRNA method for computational identification miRNAs from Acyrthosiphon pisum, Pea aphid, belonging to order Hemiptera and superfamily Aphidoidea. We validate the newly identified miRNAs using MiPred for the classification of real and pseudo miRNAs. We thus report 10 newly identified miRNAs, of which 9 are real precursors and mir-1923 being pseudo precursor. 1 of 10 miRNAs has 96.43% identity, 4 between 90 – 94.9% identity, 2 between 85–89.9% identity and 3 of less than 85% identity percent. The statistical sequence characteristics are defined for all 10 miRNAs. We further observe that U being a predominant at end base in mature sequence. We conclude thus, our study to be the beginning of understanding the A. pisum genome and may play an important role in effective pest management in near future.
Invertebrates, Insect, Base frequency, real miRNA, pseudo miRNA
