1Department of Veterinary Medicine, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner
2National Research Centre on Camel, Jorbeer, Bikaner, India
Department of Veterinary Parasitology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner
*Email: bhavanarathoredr@gmail.com
Online published on 2 December, 2013.
A molecular study was carried out to isolate the defensin gene of Hyalomma dromedarii of camels, which is a part of innate immune response of the tick. Engorged adult H. dromedarii ticks (n=350) were collected from camel herds in the Bikaner district of Rajasthan. Total genomic DNA was isolated from the salivary glands of ticks. Primers were designed for amplification of defensin gene from H. dromedarii by using base sequence of Ixodes ricinus. The defensin gene of H. dromedarii was successfully amplified from genomic DNA and was identified on the basis of its size in agarose gel electrophoresis as 580 bp.
Camelus dromedarius, defensin gene, Hyalomma dromedarii, polymerase chain reaction, salivary gland
