Molecular survey for the presence of Corynebacterium pseudotuberculosis from sheep and camel lymph nodes by PCR in Iran

The carcasses of sheep (500) and camel (150) slaughtered for human consumption as food were examined for the presence of pseudotuberculosis (Caseosus Lymphadenitis abbreviated - CLA) lesions in the Najafabad Slaughterhouse, Isfahan province, Iran, during December 2012-March 2013. Also, a total of 150 camel carcasses were examined. The lymph nodes were forwarded to the laboratory for analysis. Abscessed lymph nodes (prescapular) were taken from 78 carcasses from both (41 sheep and 37 camel). The samples were immediately transported to the Biotechnology Research Centre of Islamic Azad University of Shahrekord Branch laboratory. PCR method was used to isolate C. pseudotuberculosis from 11 out of 78 abscessed lymph nodes collected in 78 animals, with 4/37 isolated in camel and 7/41 in the sheep, displays the infections in the different sex categories, but predominant positive cases were in the female animals in both the species.