Isolation and molecular identification of the lactic acid bacteria from raw camel milk

Morphological and biochemical characterisation of lactic acid bacteria (LAB) were conducted to identify 2 bacterial strains from camel milk. Two isolates of lactic acid bacteria were identified by PCR amplification and sequencing of a fragment of 16S rRNA. The sequences obtained from both isolates were compared to those of reference strains held in Gene Bank. A similarity of 95% was considered to provide identification of these isolates to be related to the genus Enterococcus. A confirmation of identification was obtained when a relationship between these isolates and other Enterococcus taxa was constructed. Neighbour-joining method provided somewhat accurate identification of the 2 Enterococci isolates obtained from camel milk.