Journal of Camel Practice and Research

SCOPUS
  • Year: 2014
  • Volume: 21
  • Issue: 2

Isolation and molecular identification of the lactic acid bacteria from raw camel milk

  • Author:
  • Samy Farouk Mahmoud1,2, Metwally Mohamed Montaser1,3, Othman AL Zhrani1, Sayed Amin M. Amer4,5,
  • Total Page Count: 7
  • Page Number: 267 to 273

1Department of Biotechnology, Taif University, Taif, Kingdom of Saudi Arabia

2Departement of Special Food and Nutrition, Food Technology Research Institute, Agricultural Research Centre, Giza, Egypt

3Department of Zoology, Faculty of Science, AL-Azhar University, Egypt

4Department of Biology, Faculty of Science, Taif University, Taif, Kingdom of Saudi Arabia

5Department of Zoology, Faculty of Science, Cairo University, Egypt

Abstract

Morphological and biochemical characterisation of lactic acid bacteria (LAB) were conducted to identify 2 bacterial strains from camel milk. Two isolates of lactic acid bacteria were identified by PCR amplification and sequencing of a fragment of 16S rRNA. The sequences obtained from both isolates were compared to those of reference strains held in Gene Bank. A similarity of 95% was considered to provide identification of these isolates to be related to the genus Enterococcus. A confirmation of identification was obtained when a relationship between these isolates and other Enterococcus taxa was constructed. Neighbour-joining method provided somewhat accurate identification of the 2 Enterococci isolates obtained from camel milk.

Keywords

Arabian, 16S rRNA, Enterococcus, lactic acid bacteria, PCR