Capsular typing of Staphylococcus aureus isolates from camel and other domestic animals using duplex polymerase chain reaction

The present study reports successful development of duplex PCR to detect cap5K and cap8K genes in single PCR reaction in 102 S. aureus isolates obtained in our study. The cap5K gene was detected in 54 (52.94%) isolates with single amplicon of 361bp, cap8K in 33 (32.3%) isolates with 173bp amplicon, both genes were detected in 12 (11.76%) isolates with both amplicons whereas 3 isolates (one each from dog, buffalo and cattle) were nontypeable. Among 8 camel isolates, 5 were found to be positive for cap5K gene, 2 isolates were positive for cap8K gene and one isolate with both genes.