Detection of genetic variations among Staphylococcus aureus associated with camel skin wounds on the basis of spa and coa Gene

The present study was carried out to determine genetic variations among 26 isolates of Staphylococcus aureus obtained from camel skin wounds. The variations were detected by polymorphisms pattern of protein A (spa) and coagulase (coa) gene including RFLP of coa gene. Twenty five isolates produced seven different spa types (amplicon size varied between 160 and 300 bp) while one isolate did not show amplification of the gene. Of the 25 spa gene positive isolates, 24 isolates produced only single amplicon whereas, one isolate produced two amplicons of 260 bp and 300 bp size. All the isolates produced single coa gene amplicon of variable size viz. 510 bp, 600 bp, 680 bp, 710 bp and 760 bp size. Six RFLP patterns were detected after digestion with AluI restriction endonuclease enzyme. Among different RFLP patterns, PI and PIII were found genetically closer while PVI was most distant.