Journal of Camel Practice and Research
SCOPUS
  • Year: 2020
  • Volume: 27
  • Issue: 2

Bioinformatics of pyrimidine metabolism in camels and Trypanosoma evansi: Uridine 5'-Diphosphate (UDP) Metabolic pathways and targeting atp diphosphatase

  • Author:
  • Mahmoud Kandeel1,3,*, Abdulhafez Dalab2, Abdulkarem Al-Shabebi2, Abdulla Al-Taher1
  • Total Page Count: 14
  • Published Online: Dec 23, 2020
  • Page Number: 145 to 158

1Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Alahsa, 31982, Saudi Arabia

2Department of Anatomy, College of Veterinary Medicine, King Faisal University, Alahsa31982, Saudi Arabia

3Department of Pharmacology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 35255, Egypt

*SEND REPRINT REQUEST TO MAHMOUD KANDEEL email: mkandeel@kfu.edu.sa

Abstract

Comparison of host and parasite genomes can lead to discovery of metabolic or structural differences that can be used in finding new drug targets. In this study, the pyrimidine metabolic pathway uridine 5'-diphosphate (UDP) and deoxyuridine 5-diphosphate (dUDP) paths were researched. In this perspective, several UDP/dUDP metabolising enzymes were investigated by bioinformatics tools. UMP/CMP kinase was not detected in Trypanosoma evansi (T. evansi) allowing ATP-diphosphatase as an important target for inhibition studies as the interconversion between UMP and UDP are affected. While 100% similarity rate was evident in camel species, there was about 30% differences between the camel and human ATP-diphosphatase. The great sequence differences between the camel and T. evansi ATP-diphosphatases (73% differences) suggests for vulnerability of using this target for future studies. However, further investigations are required to establish the biological aspects of the enzyme.

Keywords

Camel, CTP synthase, Genome, Nucelotide, Trypanosoma evansi, UDP