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¶Co-first author: Bin Yang¶ and Lihui Chen
The transcriptome alterations in renal tubular cells of Bactrian camel treated with high glucose were investigated. Primary tubular epithelial cells, obtained from kidney of Bactrian camel were treated with low glucose (LG) or high glucose (HG). The transcriptome gene expression in primary tubular epithelial cells of Bactrian camel was examined using RNA sequencing technology. 1322 genes from HG and LG were significantly altered. The up- regulated genes in the HG group include CAMK2B, CRYAA and VNN1. Several pathways associated with diabetic nephropathy including PI3K-Akt signaling pathway, AGE-RAGE signaling pathway, ECM-receptor interaction and PPAR signaling pathway were activated by HG stimulation. This study provides scientific basis for understanding the mechanism of renal tolerance to hyperglycemia of Bactrian camel.
Bactrian camel, High glucose, Transcriptome, Tubular epithelial cells