Journal of Camel Practice and Research
SCOPUS
  • Year: 2021
  • Volume: 28
  • Issue: 3

Transcriptome Analysis of Renal Tubular Epithelial Cells of Bactrian Camel in Response to Hyperosmotic Stress

  • Author:
  • Bin Yang1, Lihui Chen1, Dong Ai2, Lu Chu3, Changmei Wang4, Zhuwei Guo1, Zhiyong Li5, Tana Han6, Demtu Er1
  • Total Page Count: 4
  • Published Online: Dec 30, 2021
  • Page Number: 245 to 248

1College of Veterinary Medicine, Inner Mongolia Agricultural University, Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, Ministry of Agriculture and Rural Affairs of P. R. China, Hohhot, Inner Mongolia Autonomous Region, 010011, China

2Agriculture and Animal Husbandry Bureau of Alxa League, Bayanhot, 750306, China

3Brigade of Alxa Left Banner agriculture and animal husbandry comprehensive administrative law enforcement, Bayanhot, 750300, China

4Supply and Marketing Cooperative Union, Ejina Banner, Alxa League, Dalaihub, 735400, China

5Detachment of Alxa League Agriculture and Animal Husbandry Comprehensive Administrative Law Enforcement, Bayanhot, 750306, China

6Comprehensive Security and Technology Promotion Centre of Dalaihub Town, Ejina Banner, Alxa League, Inner Mongolia Autonomous Region, 735400, China

SEND REPRINT REQUEST TO CORRESPONDING AUTHOR DEMTU ER* email: eedmt@imau.edu.cn

Abstract

The transcriptome alterations in renal tubular cells of Bactrian camel treated with NaCl hyperosmotic treatment were investigated. Primary tubular epithelial cells, obtained from kidney of Bactrian camel were treated with medium supplemented with NaCl to a total final osmolality of 600 mOsm. The transcriptome gene expression in primary tubular epithelial cells of Bactrian camel was examined using RNA sequencing technology. 5,114 genes from NaCl supplementation (600 mOsm) and control (300 mOsm) were significantly altered. The up-regulated genes in the 600 mOsm group include solute carrier family, ATP-binding cassette family, sodium channel, potassium channel, calcium channel, Na+/K+ ATPase, aquaporin, cytochrome P450 and heat shock protein. Some genes are associated with Bactrian camel’s salt tolerance. This study will provide scientific basis to understand the mechanism of Bactrian camel’s tolerance to high salt.

Keywords

Bactrian camel, Salt tolerance, Transcriptome, Tubular epithelial cells