1Department of Clinical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa31982, Al-HufofP.O. 400, Kingdom of Saudi Arabia
2Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza12515, Egypt
3Ministry of Agriculture, Kingdom of Saudi Arabia
4Veterinary Serum Research Institute, Cairo-Egypt
5Department of Physiology, Biochemistry and Pharmacology (Biochemistry), College of Veterinary Medicine, King Faisal University, Al-Ahsa, 31982, Al-Hufof, P.O. 400, Kingdom of Saudi Arabia
6Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Egypt
7Central Diagnostic Lab., College of Veterinary Medicine, King Faisal University, Al-Ahsa, 31982, Al-HufofP.O. 400, Kingdom of Saudi Arabia
8Physiology Department, Plant Protection Research Institute (PPRI), Agricultural Research Centre (ARC), Giza, Egypt
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Online published on 14 April, 2022.
A total of 42 infertile male dromedary camels (4-12 years old, 500-800 kg) and 7 young male camels (control, 2-2.5 years old) were enrolled in the present study. All camels were sedated and preputial swabs were collected and immediately immersed in 1 ml sterile solution of 0.9% NaCl. Preputial swabs were transferred refrigerated within 1 hour to the bacteriological laboratory. After preputial swabbing, semen samples were collected from infertile camels using an electro-ejaculator and evaluated for routine semen quality parameters using the conventional methods. Statistical analyses were conducted by Student’s t-test and Chi-square (X2). Results revealed that semen parameters were reduced in infertile camels with preputial contamination. The bacterial count in 1 ml swab sample was 148x103 and 0.24x103 in infertile mature camels and control immature camels, respectively. Nine bacterial species isolated from 49 camels’ preputial swabs comprised 41 gram-positive species (83.7%) and 8 gram-negative species (16.3%). The swabs of infertile camels had colonies species of Staphylococcus, Streptococcus, Corynebacterium, Bacillus, E. coli, Pseudomonas aeruginosa, Actinomyces, Fusobacterium, Clostridium perfringens at percentages of 35.72% (n=15), 21.43% (n=9), 11.91% (n=5), 9.52% (n=4), 7.14% (n=3), 4.76% (n=2), 4.76% (n=2), 2.38% (n=1), 2.38% (n=1), respectively. Candida colonies appears in concurrence with bacterial colonies in 28.57% (n=12). In control camels, the examined samples had colonies of Streptococcus spp., Bacillus spp. and E. coli spp. at proportions of 42.86% (n=3), 42.86% (n=3), 14.28% (n=1), respectively. Candida spp. observed in 5 colonies (71.43%) of control camels. In conclusion, Staphylococcus, Streptococcus and Bacillus were the frequently isolated bacterial spp. from prepuce of camel.
Bacterial diversity, Camels, Fungus, Prepuce
