Journal of Camel Practice and Research
SCOPUS
  • Year: 2022
  • Volume: 29
  • Issue: 3

Characterisation of Salivary Gland Proteins and P-18 Gene of Camel Ticks from Bikaner

  • Author:
  • H.K. Changal1,*, R.K. Purohit2, G. Nagarajan3, P.N. Sivalingam
  • Total Page Count: 5
  • Published Online: Dec 26, 2022
  • Page Number: 359 to 363

1Maharaja Ganga Singh University, Bikaner, Rajasthan, India334001

2Radiation Biology Laboratory, Department of Zoology, Govt Dungar College, Bikaner

3National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner, Rajasthan, India

*SEND REPRINT REQUEST TO H.K. CHANGAL email: changalharish@gmail.com, changalharish@rediffmail.com

Online Published on 26 December, 2022.

Abstract

The main objective of this study was to investigate the various salivary gland proteins and gene analysis of camel ticks Hyalomma dromedarii found on one-humped camels (Camelus dromedarius) in Bikaner city, Rajasthan, India. For this purpose, Salivary glands were collected from 5 adult ticks (engorged females). Various protein analysis from salivary gland using SDS-PAGE for determination of protein bands and their molecular weights for each fraction was done. The molecular weight of resolved major bands ranged from 14.4 to 96.0 KDa. Out of them large majority of protein molecular weight was around 18 KDa. This low molecular weight band of around 18 KDa was detected in the all 5 fractions of salivary gland of Hy. dromedarii ticks. The protein gene P–18 of Hy. dromedarii of present study had 90.4% sequence identity with that of Hy. asiaticum from China.

Keywords

Camel Hyalomma dromedarii, P-18 Gene salivary gland proteins