Journal of Camel Practice and Research

SCOPUS
  • Year: 2023
  • Volume: 30
  • Issue: 2

Dose Specific Effects of Ionomycin on Parthenogenetic Activation of in vitro Matured Dromedary Oocytes

  • Author:
  • Mohammad Shamim Hossein1,†, Young-Bum Son2,†, Yeon Ik Jeong1, Mina Kang1, Huijeong Kim1, Yura Bae1, Han Seock Kim1, Ji Young Noh1, Kyung Ik Hwang1, Woo Suk Hwang1,3,*
  • Total Page Count: 6
  • Published Online: Aug 16, 2023
  • Page Number: 185 to 190

1UAE Biotech Research Centre, P.O. Box 30310, Al Wathba South, Abu Dhabi, United Arab Emirates

2Department of OBS/Theriogenology, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea

3Department of Biology, North-Eastern Federal University, Yakutsk, 67707, Sakha Republic, Russia

Abstract

Evaluation of the dose-specific effects of ionomycin on the parthenogenetic activation of Camelus dromedarius oocytes was done in the present study. Ovaries were collected from a local slaughterhouse. Cumulus-oocyte-complexes (COCs) were aspirated and cultured in a commercial IVM medium (BO-IVM) for 42 hrs in a humidified atmosphere containing 5% CO2 at 38°C. All metaphase II oocytes were activated either 2.5 μM, 5.0 μM or 10.0 μM of ionomycin for 4 min. Activated oocytes were immediately incubated in 2.0 mM 6-dimethylaminopurine (6-DMAP) in a commercial embryo culture medium (BO-IVC) for 4 hrs at 39°C in a humidified incubator with 5% CO2. Oocytes were cultured in BO-IVC for 7 days and developmental stages were monitored and recorded. After 42 hrs of in vitro maturation, 71.28% of oocytes were found with the extruded first polar body (metaphase II oocytes). The oocytes in the 5.0 μM of ionomycin group showed the highest blastocyst formation rate (56.99%) compared to the 2.5 μM (6.58%) and 10.0 μM (1.81%) groups. We recommended 5.0 μM of ionomycin for 4 min followed by incubation in 2.0 mM 6-DMAP for 4 hrs to activate camel oocytes.

Keywords

Camel, In vitro maturation, Ionomycin, Oocytes, Parthenogenetic