1Department of Animal Genetics and Breeding, College of Veterinary Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334001, Rajasthan, India
2Department of Livestock Production Management, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
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Online Published on 25 December, 2023.
The aim of the present study was to investigate the genetic and phylogenetic analysis of kappa casein gene in the 70 Bikaneri breed of camels which were blood sampled for isolation of their DNA. The polymerase chain reaction (PCR) products (488bp) of kappa casein gene were sequenced using the Sanger dideoxy chain termination method. The sequence analysis of kappa casein gene revealed presence of within-species variation. Intraspecies variation within camel were observed in the form of four SNPs. Generated Sequences were submitted on NCBI GENBANK. Furthermore, a Neighborhood Joining Phylogenetic tree was constructed through MEGA7 bioinformatics software. The sequencing of the kappa casein gene in Bikaneri camel may be useful for future studies on genetic variation within and between camel populations or on associated traits due to the therapeutic importance of camel milk for human consumption.
Camel, Kappa casein, Milk, Polymorphism, Snp
