Soybean Processing and Utilization Centre, Central Institute of Agricultural Engineering, Nabi bagh, Berasia Road, Bhopal-462 038, India.
Cellulase and papain were used for oil recovery from soybean during mechanical pressing independently as well as in combination. Enzyme ratio of 50:1 for papain and of 5:1 for cellulase were employed for hydrolysis. Three incubation period (2, 4 and 24 h) with constant shaking were used. The incubation temperature (25°C), moisture content of 3% w.b and a pressing time of 60s were constantly maintained during mechanical processing. The treated samples were compressed uniaxially in a test cell at 20, 35 and 50 MPa and the oil recovery was monitored. Papain and cellulase treatment increased 6.0 and 14.8% oil recovery while their combination was ineffective. The optimum pressure was found to be 50 MPa and the incubation period 4 h.
