Department of Dairy Microbiology, Dairy Science College, Karnataka Veterinary Animal, Fisheries Sciences University, Hebbal, Bangalore-560 024, India.
To overcome the problems associated with liquid culture techniques for the production of cell concentrates, an SSF technique using inert substrate such as paddy husk with various additives were attempted to standardize the SSF medium. The standardized SSF medium was used to produce cell concentrate of starter cultures using Bifidobacterium longum F8. The results showed that viable counts of 10.85 log10 cfu/g of B. longum F8 could be achieved when medium was standardized with various growth promoting nutrients with the incubation conditions. The cultures which were grown on the SSF medium were preserved by air drying which was resulted in a reduction of cells up to one log cells per gram and retained minimum viability on storage at 30°C. The dried SSF cultures were evaluated for their performance in milk. The results of viable count, curdling time, acidity shown that the results were comparable with the control (liquid culture).
Solid state fermentation, Starter cultures, SSF cultured medium, Paddy husk
