1Fortis Escort Hospital, Majitha Road, Amritsar-143 004
2Department of Food Technology, Guru Jambheshwar University of Science & Technology, Hisar-125001
Department of Biochemistry, CCS Haryana Agricultural University, Hisar-125 004, India
*Corresponding author, 410, Sector-15A, Hisar-125001
Online published on 13 January, 2015.
Absorption of glucose, sucrose, glutamic acid, methionine, lysine and cysteine was studied using the intestinal everted sac technique. Rats were fed rice diets Basmati 370, Pusa Basmati 1 and Haryana Basmati 1 using as a casein control. Absorption of nutrients was monitored spectrophotometrically and of glucose, sucrose and glutamic acid by 14c radio assay using scintillation counting. Positive correlation coefficients between the two assay methods for glucose (r = 0.84), sucrose (r = 0.38) and glutamic acid (r = 0.80) were observed. Glucose showed higher absorption than sucrose and glutamic acid than lysine, cysteine and methionine. Absorption of glucose, sucrose and glutamic acid were higher in rats fed on Haryana Basmati 1 diet as demonstrated by both the methods. Rats on Basmati 370 diet exhibited higher methionine, lysine and cysteine absorption. Absorption of nutrients differed significantly between casein and rice.
Amino acids, Intestinal absorption, Radio assay, Rice, Spectrophotometric technique
