Asian Journal of Dairy and Food Research
SCOPUS
  • Year: 2022
  • Volume: 41
  • Issue: 2

Association of SSCP polymorphisms of HSP70 gene with physiological, production and reproduction performance in sahiwal and crossbred cows

  • Author:
  • J. Sai Prasanna1,*, S.T. Viroji Rao1, M. Gnana Prakash1, Suresh Rathod2, P. Kalyani3, B. Rajith Reddy1
  • Total Page Count: 6
  • Page Number: 150 to 155

1Department of Animal Genetics and Breeding, College of Veterinary Science, Rajendranagar, Hyderabad-500 030, Telangana, India

2Department of Livestock Farm Complex, College of Veterinary Science, Rajendranagar, Hyderabad-500 030, Telangana, India

3Department of Veterinary Biotechnology, College of Veterinary Science, Rajendranagar, Hyderabad-500 030, Telangana, India

*Corresponding Author: J. Sai Prasanna, Department of Animal Genetics and Breeding, College of Veterinary Science, P.V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad-500 030, Telangana, India. Email: drsaiprasannareddy@gmail.com

Online published on 15 July, 2022.

Abstract

Cellular tolerance to heat stress is mediated by heat shock proteins (HSPs). The HSPs act as molecular chaperones and are transcribed in response to stress. Among different families of these proteins, HSP70 is considered to be related to the development of temperature tolerance. Unraveling polymorphism in heat shock protein genes could be a step towards the identification of genetic markers for selecting heat-tolerant cattle.

The present study was carried out in Sahiwal (n=50) and Crossbred cows (n=50) with the objective to identify polymorphisms in HSP70 gene. Two fragments (295 and 220 bp) of HSP70 gene were subjected to Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique. Statistical analysis was performed to study the association of each SSCP genotype on physiological, production and reproduction traits in Sahiwal and crossbred cows using the univariate GLM model of SPSS 25.

The PCR-SSCP of 295 bp fragment of HSP70 gene revealed two genotypes AA and AB in Sahiwal cows and two genotypes AA and AC in crossbred cows. The association analysis revealed that genotype AA had higher peak milk yield in Sahiwal cows while the same genotype had higher total lactation milk yield, lower service period and calving interval in crossbred cows. The 220 bp fragment was found to be monomorphic in both Sahiwal and crossbred cows.

Keywords

Crossbred cows, Heat stress, HSP70, Polymorphisms, Sahiwal