1Department of Livestock Products Technology (Meat Science), Madras Veterinary College, Chennai-600 007, Tamil Nadu, India.
2College of Food and Dairy Technology, Koduveli, Chennai-600 051, Tamil Nadu, India.
3Department of Pharmacology and Toxicology, Madras Veterinary College, Chennai-600 007, Tamil Nadu, India.
4Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Chennai-600 051, Tamil Nadu, India.
*Corresponding Author: V. Govind, Department of Livestock Products Technology (Meat Science), Madras Veterinary College, Chennai-600 007, Tamil Nadu, India, Email: govindvet@gmail.com
Online Published on 05 April, 2023.
Enrofloxacin used considerably on large scale among poultry birds. Detection and monitoring of its residues is vital in the domestic and export market. A simple and rapid LCMS/MS method for screening of Enrofloxacin and its metabolite residues in poultry liver samples was described.
Target analytes were extracted with a solvent combination of 5% phosphoric acid in 50% acetonitrile in water. It was followed by deep freezing for efficient separation of the liquid phase. Later extractants were subjected to centrifugation at 4°C, 10,000 rpm for 20 minutes and supernatant was filtered and injected into LCMS/MS. 0.1% formic acid is used as mobile phase additive for both aqueous and organic mobile phase acetonitrile.
Accuracy and precision values of the method are within the acceptable limit of (<5% RSD) accorded by the European commission. Standardized method has been applied to determine the Enrofloxacin and Ciprofloxacin residues in liver samples collected in local markets and found within maximum residue limits.
Chicken liver, Ciprofloxacin, Enrofloxacin, LC-MS/MS, Low temperature partitioning, Residues
