Asian Journal of Dairy and Food Research
SCOPUS
  • Year: 2024
  • Volume: 43
  • Issue: 2

ELISA based monitoring and quantification of tetracycline residues in fresh and powdered cow milk commercialized in Constantine region (Northeast Algeria)

  • Author:
  • L. Boultif1,*, N. Zeghilet2, M.C. Abdeldjelil1, A. Boudebza1, B. Chebira1
  • Total Page Count: 7
  • Page Number: 306 to 312

1PADESCA Research Laboratory, Institute of Veterinary Sciences, Mentouri Constantine 1 University, Constantine, Algeria

2GSPA Research Laboratory, Institute of Veterinary Sciences, Mentouri Constantine 1 University, Constantine, Algeria

*Corresponding Author: L. Boultif, PADESCA Research Laboratory, Institute of Veterinary Sciences, Mentouri Constantine 1 University, Constantine, Algeria, Email: boultif.latifa@umc.edu.dz

Online published on 5 July, 2024.

Abstract

This study aimed to identify tetracycline residues in milk consumed in the region of Constantine (northeast Algeria) using ELISA based tetracyclin kit.

A total of 180 samples were analyzed (fresh cow milk and imported powdered milk). To compare ELISA and HPLC detection values, 22 fresh milk ELISA positive samples were confirmed by HPLC analysis.

92.5% of fresh milk samples contained tetracycline residues at values between 5 and 74 μg/L and 33.3% of the samples showed concentrations between 49 and 74 μg/L, that exceed the MRLs recommended by the FDA. No significant differences (p>0.01) were found between the values obtained by the two methods.

Keywords

Antibiotic residues, ELISA, Fresh milk, Powdered milk, Tetracycline