Asian Journal of Dairy and Food Research
SCOPUS
  • Year: 2025
  • Volume: 44
  • Issue: 5

Physiological and Histological Ameliorative Effects of Walnutspulp Extract on Kidney Function of Rats Treated with Citalopram

  • Author:
  • Mutashar Jaddoa Jiheel1, Alwan Al Qushawi2, Ahmed Dhahir Latif3, Hasanain A.J. Gharban4,*
  • Total Page Count: 8
  • Page Number: 753 to 760

1Department of Medical Analysis, College of Science, University of Waist, Wasit, Iraq

2Department of Basic Science, College of Dentistry, University of Waist, Wasit, Iraq

3Department of Pharmacology and Toxicology, College of Medicine, University of Waist, Wasit, Iraq

4Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Wasit, Wasit, Iraq

*Corresponding Author: Hasanain A.J. Gharban, Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Wasit, Wasit, Iraq, Email: hghirban@uowasit.edu.iq

Online published on 30 October, 2025.

Abstract

Citalopram is a class of antidepressant drugs that works by increasing the amount of serotonin to maintain mental balance. However, the risk of kidney damage associated with Citalopram use remains an area of ongoing research, with several potential mechanisms implicated in its pathogenesis. This experimental study aims to evaluation of physiological and histological renal damages due to low and high doses of Citalopram and the ameliorative role of walnuts-pulp extract in reducing nephrotoxic damages.

An overall 28 mature albino male rats were purchased, acclimated and divided equally and randomly into four groups; NG given distilled-water only, EG1 given Citalopram (0.6 mg/kg.BW) and walnut-pulp extract (10 mg/kg.BW), EG2 given Citalopram (20 mg/kg.BW) and walnut-pulp extract (10 mg/kg.BW) and PG given Citalopram only (0.6 mg/kg.BW). Blood was sampled from all animals in the day-1 and day-60 of experiment; while, renal tissues were collected after the ending of experiment (60 days).

Our findings showed that the concentrations of blood urea and creatinine at day-1 were differed insignificantly (p>0.05) among study groups; however at day-60, significant elevation in concentration of urea was seen in EG2 and PG; while, increases in concentration of creatinine was found in EG2 when compared to other study groups. In comparison between the values of study groups at day-1 and day-60, significant increases were identified in concentration of urea of EG2 and PG; as well as creatinine of EG2 at day-60. In tissue sections of NG, EG1 and EG2; the results of light microscopy at 100X and 200X revealed the presence of normal structures in tubules and glomeruli without any significant occupied lesions. In tissue sections of PG, the results of light microscopy at 100X and 200X showed clearly the presence of renal vein congestion with excessive perivascular leukocyte cuffing and infiltration of homogenous and pinkish materials. Also, there was a narrowing in the renal artery diameter and glomeruli, with presence of atrophy characteristics in the glomerular tuft.

Keywords

Antidepressant, Creatinine, Juglans regia, Nephrotoxicity, Urea