1National Center of Hematology, Mustansiriyah University, Baghdad, Iraq
2Department of Microbiology, Collage of Medicin, Mustansiriyah University, Baghdad, Iraq
3Department of Pharmacology and Toxicology, College of Pharmacy, Mustansiriya University, Baghdad, Iraq
4Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq
5Department of Biology, Al-Farabi University College, Baghdad, Iraq
Polycythemia vera (PV) is a myeloproliferative neoplasm characterized by the overproduction of red blood cells. A key feature of PV is the dysregulation of cytokine networks, with elevated levels of various pro-inflammatory cytokines contributing to disease progression. The JAK2 mutation, a common genetic driver of PV, stimulates the production of these cytokines, leading to increased cell proliferation, inhibited apoptosis and inflammation. Estimation of IL-12 and IL-21 Level and study the correlation between of theses cytokine with JAK2 V617F and hematological parameters.
This study investigated 60 patients diagnosed with polycythemia vera (PV) at the National Center of Hematology between January 2023 and July 2024. The patient group consisted of 33 females and 27 males, with ages ranging from 18 to 75 years. Detection JAK2 V617F using Poly chain reaction (PCR) as well as estimation of IL-12 and IL-21 by sandwich ELISA technique.
In this Study 31 of PV patients detected as JAK2 V 617F Negative while 29 patients were positive. Also, this study examined the role of IL-12 and IL-21 in polycythemia vera (PV). Patients with the JAK2 V617F mutation had significantly higher IL-12 levels, suggesting a strong association. However, no significant differences were found in IL-21 levels between groups. Additionally, WBC count and platelets count were positively correlated with IL-21.
Il-12, Il-21, Jak2 V617f, Polycythemia vera
