1Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia
2Faculty of Veterinary Medicine, Universitas Syiah Kuala, Jl. Tgk. Hasan Krueng Kalee No. 4, Kopelma Darussalam, Kota, Banda Aceh, 23111, Aceh, Indonesia
3Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia
4Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Jl. Raya Bogor Km. 46 Cibinong, Bogor, 16911, West Java, Indonesia
5Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, 480211, Nigeria
*Corresponding Author: Mirni Lamid, Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya, 60115, East Java, Indonesia, Email: mirnylamid@fkh.unair.ac.id
Online Published on 10 March, 2026.
The KUB chicken industry faces several problems in early growth and active biological activity. One thing that needs to be done is the addition of phytase and protease enzymes to livestock feed. One effort to increase other livestock production is through molecular or genetic markers. The purpose of this study was to determine the effect of adding phytase and protease enzymes to the ration on the performance of KUB chickens, as well as to determine the expression of the MSTN gene on KUB chicken carcass production.
A total of 35 blood samples were taken from superior Balitnak (KUB) native chickens, consisting of 8 blood samples from groups of male chickens with the same variety, age with feed added with phytase enzyme, samples were taken from male chickens with the same body weight given feed with a mixture of protease enzymes. The following methods were used to calculate body weight increase, feed intake and feed conversion ratio (FCR). Amplification of the myostatin gene (MSTN) in KUB chickens was carried out using the Polymerase Chain Reaction (PCR) technique.
Phytase and protease enzyme supplementation did not show a significant effect (P>0.05) on the relative weight of chicken internal organs, but has the potential to increase digestive efficiency and metabolic function. Enzyme activity in the digestive tract is considered an important factor that can affect gut health and nutrient digestibility. MSTN gene transcript expression was detected in all enzyme doses investigated in this study. Myostatin (MSTN) gene expression in KUB chickens was associated with muscle development, growth rate, organ composition and body weight.
Agriculture, KUB chicken, MSTN, Phytase, Protease
