1Institute of Medical Technology, Middle Technical University, Baghdad, Iraq
2Department of Radiology Techniques, Dijlah University College, Baghdad, Iraq
3Department of Biotechnology, College of Science, University of Baghdad, Baghdad, Iraq
4Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq
5Department of Biology, Al-Farabi University College, Baghdad, Iraq
6Department of Marine Biology, Faculty of Marine Sciences, King Abdulaziz University, Saudi Arabia
*Corresponding Author: Ahmed Flayyih Hasan, Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq, Email: ahmed_flayyih@nahrainuniv.edu.iq
Online Published on 10 March, 2026.
This study focused on Iron oxide nanoparticles (Fe2O3-NPs) from multiple concentrations of Iron oxide nanoparticles (Fe2O3-NPs) on oxidation of lipids, the defence system of antioxidants and biochemical measurements in the liver of male mice.
Twenty male Albino Mice weighing 25-30 g were used in the study. Animals were categorised into four groups, each comprising 10 mice. The initial group served as the control. Groups II, III and IV received oral administration of Tin oxide nanoparticles at 50, 25 and 10 mg/kg weight body per day for four weeks. Blood and liver samples were collected after the experimental period to investigate various parameters.
Treatment with Iron oxide nanoparticles (Fe2O3-NPsin) varying concentrations elevated levels of in comparison to the control group, the liver and kidneys exhibited reduced glutathione (GSH) content, glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S-transferase (GST) activities, as well as hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances (TBARS). The protein contents of rats were significantly reduced when they were administered iron oxide nanoparticles (Fe2O3-NPs) at concentrations that differed from those of the control group. Treatment with iron oxide nanoparticles (Fe2O3-NPs) at varying concentrations substantially increased urea and creatinine. In mice, an increase in urea and creatinine concentration is a substantial indicator of liver dysfunction. In mouse liver homogenates, the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were significantly reduced when treated with iron oxide nanoparticles (Fe2O3-NPs) at varying concentrations. Conversely, lactate dehydrogenase (LDH) was significantly increased. It is clear that iron oxide nanoparticles (Fe2O3-NPs) induce pronounced hazardous effects in the liver of mice in a dose-dependent manner, Biomarkers for the detrimental effects of iron oxide nanoparticles (Fe2O3-NPs) may include estimating lipid peroxidation, enzymatic and non-enzymatic antioxidants and biochemical parameters.
ALT, Fe2O3-NPs, GPx, Male mice, SOD, TBARS
