1Laboratoire de Recherche Sciences et Environnement: Bioressources, Géochimie-Physique Législation et Développement Socio-Economique, Department of Life Sciences, Faculty of Sciences and Technology, University of Tamanghasset, BP 10034-Sersouf, Tamanrasset- Algeria
2Department of Animal Science, Institute of Animal Science, Biotechnology and Natural Conservation, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary
3Institute of Animal Sciences and Wildlife Management, Faculty of Agriculture, University of Szeged, Hódmezõvásárhely, Hungary
4Doctoral School of Animal Science, University of Debrecen, Debrecen, Hungary
*Corresponding Author: Habiba DRICI, Laboratoire de Recherche Sciences et Environnement: Bioressources, Géochimie-Physique Législation et Développement Socio-Economique, Department of Life Sciences, Faculty of Sciences and Technology, University of Tamanghasset, BP 10034-Sersouf, Tamanrasset- Algeria, Email: dricih11@gmail.com
Online Published on 10 March, 2026.
The development of an optimal culture medium for microbiological analysis is a significant advancement in the field. The conventional PCA for RAMF enumeration may not accurately assess raw camel milk’s microflora, potentially compromising food safety evaluations. Our study addresses this crucial issue. We developed CaM-PCA medium using sterilized whole camel milk and compared it with standard PCA using twenty-three raw milk samples. Enumeration results were analyzed using Mann-Whitney test and exclusive isolates were identified through 16S rDNA sequencing. CaM-PCA yielded significantly higher RAMF counts (8.50±0.45 log10 CFU ml-1) than PCA (8.20±0.60 log10 CFU ml-1), P=0.048. Three isolates exclusive to CaM-PCA were identified as Metabacillus sp., Hafnia alvei and Enterobacter hormaechi subsp. hoffmannii, demonstrating CaM-PCA’s enhanced ability to detect spoilage and opportunistic pathogens in raw camel milk.
Bacterial isolation, Camel milk, Culture medium, Enterobacter, Food safety, Hafnia alvei, Metabacillus, Microbial enumeration, RAMF
