1Department of Histology and Biology, Faculty of Medicine, Universitas Islam Indonesia, Yogyakarta, Indonesia.
2Department of Surgery, Oncology Division, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
3Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
4Department of Histology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
5Department of Biochemistry, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
*Corresponding Author: Ika Fidianingsih, Department of Histology and Biology, Faculty of Medicine, Universitas Islam Indonesia, Yogyakarta, Indonesia. Email: ika_fidianingsih@uii.ac.id
Diets rich in complex carbohydrates, such as Arrowroot and diets containing casein have the potential to be effective in preventing disease. This study aims to determine whether the combination of an arrowroot diet, low sugar and added casein in oxidative stress-induced rats on body weight, consumption of feed, glucose levels, lipid profile and expression of the gene that regulates cholesterol synthesis, namely HMGCR.
The research was experimental on 1-month-old rats. A total of 20 female rats were randomized into four groups, namely 1) standard feed without induction of oxidative stress (normal group), 2) standard feed with induction of oxidative stress, 3) modified feed A (arrowroot 30% and induction of oxidative stress, 4) modified feed B (arrowroot 60% and induction of oxidative stress). After seven months, blood was collected from the orbit to examine lipid profile levels and blood glucose levels. Liver tissue was assessed for HMGCR mRNA expression.
The body weight and feed intake in the normal and modified feed B groups were higher than in the other groups. There were no significant differences between groups’ total cholesterol, LDL-C, HDL-C, triglycerides and blood glucose levels. HMGCR expression level measured as 1.05±0.38; 0.926±0.32; 1.24±0.37 and 1.55±0.6 (p=0.17), respectively.
Arrowroot, Casein, HMGCR, Lipid profile, Oxidative stress
