1Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Basrah, Basra, Iraq.
2Department of Biology, Collage of Education for Pure Sciences, University of Al-Hamdaniya, Al-Hamdaniya, Nineveh, Iraq.
3Department of Anatomy and Histology, College of Veterinary Medicine, University of Wasit, Wasit, Iraq.
4Department of Public Health, College of Veterinary Medicine, University of Basrah, Basra, Iraq.
5Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Wasit, Wasit, Iraq.
*Corresponding Author: Hasanain A.J. Gharban, Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Wasit, Wasit, Iraq. Email: hghirban@uowasit.edu.iq
Abscessed wounds in cattle represent a significant veterinary concern, often leading considerable economic losses due to decreased productivity, treatment cost, potential culling and even death. This study aims to investigate the incidence rate of Trueperella pyogenes in abscessed wounds of cattle using molecular technique with phylogenetic analysis of study isolates and estimation the relationship of infection to animal risk factors.
A total of 271 cattle of different ages and sexes admitted to private veterinarian clinics or being visited to their farms in Wasit province (Iraq) were diagnosed clinically with abscessed wounds. Then, samples of abscess swab were collected and examined molecularly by the conventional PCR. Finally, the positive T. pyogenes isolates were sequenced, submitted in the NCBI database and analysed phylogenetically.
Findings of clinical examination revealed a significant increase in abscessed wounds at abdomen but decreased in neck and back when compared to other body regions; head, forelimbs, hindlimbs and pelvis. Targeting of 16S rRNA gene, 7.38% from abscessed wounds of study cattle were positively infected with T. pyogenes. Phylogenetic analysis of the study T. pyogenes isolates revealed its identity to NCBI-BLAST USA T. pyogenes isolate (KX592206.1) at rates of similarity and mutation/changes ranged 98.47-99.86% and 0.0003-0.001%, respectively. Relation to risk factors, incidence and risk of positivity in body parts were elevated significantly in abdomen but decreased in neck and back, head, forelimbs and hindlimbs when compared to pelvis. Concerning age, although insignificant variation in incidence of positivity was seen among age groups of study animals, cattle aged 1–4 years and >4 years were appeared at higher risk of infection than those of <1 year. For sex, also insignificant differences in incidence rate of positivity was detected between females and males; however, females were detected at higher risk of infection than males.
Bovine bacterial infections, National centers for biotechnology center (NCBI), Polymerase chain reaction (PCR), Risk factors, Skin injury
