1Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2Department of Microbiology, Collage of Science, Mustansiriyah University, Iraq
*Corresponding Author: Anfal Kadhim Abed, Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. Email: anfalkadhim@uodiyala.edu.iq
Tannin exhibits potent antioxidant and anti-inflammatory properties. This study aimed to investigate the protective effects of Tannin on neuroendocrine and its correlation with hippocampal and immunological alterations in Streptozotocin-induced diabetic mice.
Sixty Adult male albino mice were divided into six groups, including control, high-dose Tannin-sole (300 mg/kg) and Streptozotocin-induced diabetic groups (150 mg/kg, intraperitoneally) treated with metformin (250 mg/kg), low-and high-dose Tannin (200, 300 mg/kg, respectively, gavage) for 28 days. Fasting blood glucose (FBG) was monitored weekly (4weeks) and serum cortisol, noradrenaline and acetylcholine levels were quantified using ELISA. Brain tissues were examined histologically to assess neuronal integrity (HandE and Cresyl Violet staining) and apoptosis (TUNEL). Spleen tissues were analyzed by HandE and Trichrome Masson staining, while, CD4+ /CD8+ T-cell distribution was determined by immunofluorescence.
Tannin treatment significantly reduced FBG in hyperglycemic mice in a time-dependent manner (p<0.05), comparable to metformin. The stress-associated biomarkers (cortisol, noradrenaline and acetylcholine) were markedly (p<0.05) reduced in Tannin-treated groups, with significant (p<0.05) reduction in noradrenaline compared to the metformin-received group. Also, significantly (p<0.05) improved pyramidal cells’ integrity and Nissl body storage and reduced apoptosis. High-dose Tannin further restored immune homeostasis in spleen tissue by attenuating CD4+ and CD8+ T-cell cellularity.
Hippocampus, Hyperglycemia, Metformin, Mice model, Spleen, Tannin
