Virulence studies and in vitro evaluation of antibiotics/antibacterial chemicals against citrus canker (Xanthomonas axonopodis pv. citri) on acid lime

The experiment, conducted to screen virulent isolate with compatible host and pathogen combination in acid lime and canker causing pathogenic culture of Xanthomonas axonopodis pv. citri (Xac) under controlled environment condition using pinprick method and the best antibiotics/antibacterial chemical to manage the disease pathogen revealed that the isolate Xac8, among the 10 isolates collected from acid lime growing areas of northern Karnataka, was the most virulent followed by Xac5. Chloramphenicol, exhibiting a minimum inhibition zone of 3.22 cm against the two highly virulent isolates of X. axonopodis pv. citri by inhibition zone assay method, was adjudged the most significant and superior antibiotics.