Screening for lignolytic enzyme activity in native lignin degrading fungi and bacteria from organic farming fields of Hyderabad and Karnataka region

The twenty five bacterial and twenty five fungal isolates were grown on selective lignin enriched medium and screened for efficient lignin degradation by tannic acid method. In this method, bacterial isolate KGSB-2 was found to be superior over the reference strain with OD value of 1.040 among bacteria and total eight bacteria were at par with reference bacterial strains and eleven fungal strains were at par with reference fungal strains. Better enzyme production was found in fungal isolates as compare to bacterial isolates. The selected eight bacterial and eleven fungal strains were subjected for series of enzymatic test mainly to know the diversity of enzyme production viz., Lignin peroxidase (LiP), Managanese Peroxidase (MnP) and Laccase activity by tannic acid method, Dye Decolouration and finally on birch wood agar medium with and without glucose. From the results obtained finally five fungal strains viz., KGST1, KGST-2, KGSA, KKSP and RMSP and two bacterial strains viz., KGSB-1 and RMSP-2 were found performing better as compared to the reference strains.