1Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
2ICAR-Central Citrus Research Institute, Nagpur-440 033, Maharashtra, India
3Department of Biological and Environmental Sciences, University of Hertfordshire, Hatfield, United Kingdom
*Authors for correspondence: kiranag0734@gmail.com
Cysts of Heteroderacajani was characterized via molecular analysis using D2-D3 regionof 28S ribosomal DNA (rDNA). Blast N analysis of the amplified fragment in NCBI database revealed that H.cajani IARI isolate showed 100% similarity with H.cajani isolate 252 (Accession No. DQ328693). The phylogenetic relations of H. cajani were evaluated by neighbour joining method. Phylogenetic tree inferred from D2-D3 region of 28S rDNA usingvarious H.cajanipopulations revealedclose relationship ofH. cajaniIARI isolate with H. cajani isolate 252.
Pigeon pea cyst nematode, Heteroderacajani, Molecular characterization, Phylogeny
