Department of Biology, Texas A & M University College Station, Texas-77843-3258 (USA).
*Address for correspondence: Biochemistry Department, Regional Research Laboratory Jorhat-785 006, Assam (India).
The effects of pyroglutamate aminopeptidase (PGA) and leucine aminopeptidase (LAP) were evaluated with the synthetic analogues, viz., allatotropin analogue-non acetylated (ATANA) and allatotropin analogue acetylated (ATAA) with female corpora allata (CA) of 3-day old Manduca sexta in vitro with and without enzyme treatment. The juvenile hormone (JH) production was measured using an in vitro radiochemical assay (RCA), monitoring the incorporation of L-[JH-methyl] methionine. The coincubation of PGA treated peptides (analogues) did not show any change in the stimulatury capacity of ATANA and ATAA due to the acetylated N-terminal (ATAA) and free-terminal (ATANA). However, leucine aminopeptidase showed considerable effect on ATANA (free-N terminal) and not on ATAA.
