1Université de Lyon, Université Lyon1, Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
Department of Sericulture & Biological Sciences, Bangalore University, Bangalore-560 056, Karnataka, India
*Corresponding E-mail: puttarajuhp@hotmail.com
Online published on 24 July, 2012.
A polymerase chain reaction (PCR) method was used to screen Wolbachia infection by using wsp (Wolbachia surface protein) primers. A nested polymerase chain reaction (standard PCR) protocol yielded false negative results in the Indian honey bees Apis millifera, Apis dorsata and Apis cerana. this may be due to lower titer of Wolbachia or to factors that interfere with amplification of Wolbachia DNA. We developed a more sensitive method of amplification based on a Long PCR protocol using a proof reading enzyme.
Wolbachia, Polymerase Chain Reaction (PCR), Standard PCR, Long PCR
