Journal of Entomological Research
SCOPUSWeb of Science
  • Year: 2017
  • Volume: 41
  • Issue: 4

Synergic effect between Bacillus thuringiensis Kurstaki HD1 δ-endotoxins and Melia azedarach (Meliacea) methanolic extract against the larvae of Thaumetopoea pityocampa Den. & Schiff. (Lepidoptera, Notodontidae)

  • Author:
  • Bachir Kamilia1,, Tail Ghania, Bouragba Nadia2, Zamoum Mohamed2, Trigui Mohamed3, Tounsi Slim3, Saidi Fairouz
  • Total Page Count: 10
  • Page Number: 343 to 352

1Biotechnology, Environment and Health's Laboratory. University Saad Dahleb Blida (USDB), 01 Soumaa's Road, P.O. Box 270, 9000 Blida, Algeria

2National institute of forest's researches. Laboratory of Forest entomology-INRF. Box 37, 16000 Cheraga, Algeria

3Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018, Sfax, Tunisia

*Corresponding author's E-mail: kamy8989@yahoo.fr

Online published on 9 January, 2018.

Abstract

Bioassays with Melia azedarach against Thaumetopoea pityocampa larvae showed positive correlation between mortality (between 50 and 100%) and the used doses (5, 10 and 15 mg/cm2) after 24 h of application. This efficacy was similar to that of the Bacillus thuringiensis Kurstaki HD1 δ-endotoxins (20 μg/ml, 40 μg/ml, 80 μg/ml, 150 μg/ml and 300 μg/ml) which resulted in a caterpillars's mortality rate of 100% but three days after application, at the dose of 300 μg/ml. The use of the mixture M. azedarach and the δ-endotoxins of Bacillus thuringiensis Kurstaki HD1 gave 100% mortality, 24 hours after treatment only. Histologically, this effect resulted in vacuolation of the intestinal cells of the treated larvae with a significant reduction of pine needle consumption compared to untreated caterpillars.

Keywords

Melia azedarach, Bacillus thuringiensis, synergic effect, biocid, Thaumetopoea pityocampa.