Mitochondrial genes and nuclear gene variation among seven species of the genus Lisarda of subfamily Salyavatinae (Heteroptera: Reduviidae: Salyavatinae)

The sequence of three mitochondrial genes viz., 16S rRNA, 28S rRNA and COII of seven Lisarda Stål species were downloaded from the GenBank and were subjected to phylogenetic analyses to understand the intrageneric and intraspecific variations and the role of geographical isolation on speciation using CLUSTAL W in MEGA version 10.0. This analysis includes seven species of Lisarda such as Lisarda annularis (Walker), Lisarda conspersa (Walker), Lisarda crudelis (Stål), Lisarda inornata (Walker), Lisarda longispina Distant, Lisarda rhypara Stål and Lisarda schweinfurthi Reuter. The pair wise genetic distances were calculated and phylograms were constructed using maximum likelihood, neighbor-joining, minimum evolution, UPGMA and maximum parsimony methods. These preliminary analyses not only demarcated the seven species of Lisarda are revealed phylogenetic relationships and the role of geographical isolation on speciation.