1Department of Entomology, College of Agriculture, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya - 224229, Uttar Pradesh, India
Department of Entomology and Agricultural Zoology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi - 221005, Uttar Pradesh, India
*Corresponding author's E-mail : ushar0056@gmail.com
Study on the biochemical basis of resistance in green gram genotypes against pulse beetle showed that highest seed damage was recorded in genotype Pant Mung-2 (69.25%) whereas, lowest seed damage was found in genotype ML-935 (46.50%). The maximum weight loss was recorded in genotype Pant Mung2 (43.00%) and minimum weight loss was recorded on ML-935 (11.67%). The lowest protein content was recorded in genotype ML-935 (11.50 mg/g) while, highest protein content was reported in genotype Samrat (27.70 mg/g) followed by Pant Mung-2 (26.90 mg/g). The genotypes Samrat (40.36 mg/g) had the highest starch content followed by Pant Mung-2 (39.00 mg/g) and genotype ML-935 (26.53 mg/g) had the lowest starch content. The genotype Samrat (8.66 mg/g) received highest sugar content followed by Pant Mung2 (7.90 mg/g) and genotype ML-935 (2.96 mg/g) received the lowest content of total soluble sugar. The genotype ML-935 (17.03 TIU/mg protein) showed highest trypsin inhibitor activity followed by ML1059 (14.50 TIU/ mg protein). The genotype Samrat (11.37 TIU/ mg protein) depicted lowest trypsin inhibitor activity followed by genotype Pant Mung-2 (11.90 TIU/ mg protein). The genotype ML-935 (29.06%) had the highest protease inhibitor activity followed by genotype ML1059 (26.50%). The genotype IPM-2-3 (17.16%) followed by genotype Ganga-8 (17.50%) showed protease inhibitor activity. A positive significant correlation was observed between per cent seed damage, per cent weight loss and biochemical parameters like protein content (r = 0.892** and r = 0.881**, respectively), starch content (r = 0.924** and r = 0.895**, respectively) and total soluble sugar (TSS) content (r = 0.886** and r = 0.855**, respectively), whereas parameters like trypsin inhibitor activity (r = -0.891** and r = -0.871****, respectively) and protease inhibitor activity (r = -0.937** and r = -0.921****, respectively) were negatively correlated.
Biochemical, Callosobruchus maculatus, Genotypes, Green gram, Pulse beetle, Resistance
