1Universite Freres Mentouri Constantine 1, Faculte des Sciences de la Nature et de la Vie, Departement de Biologie Appliquee, BP 325, Route de Ain El Bey, 25017Constantine, Algerie;
2Laboratoire de Biologie Animale Appliquee, Faculte des Sciences, Departement de biologie, Universite, Badji-Mokhtar, Annaba23000-Annaba, Algerie
Laboratoire des Systemes et Materiaux Avances, Faculte des Sciences, Departement de biologie, Universite Badji-Mokhtar, Annaba23000-Annaba, Algerie
*Corresponding author present address: E-mail: boulahbel.billel@umc.edu.dz
Online published on 18 June, 2022.
In this study, we evaluated the sublethal effects of azadirachtin on Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae) as biological model. Azadirachtin was applied topically at two doses ID25 (0.59 pg/Pupa) and ID50 (1.10 pg/Pupa) on newly ecdysed pupae. Results showed that azadirachtin reduced significantly pupal weight at the two tested doses as compared to controls. Treatment reduced, significantly eclosion rates after azadirachtin treatment, as compared to controls and the value recorded are 89.19, 72 and 50.01%, respectively for controls, ID25 and ID50. Finally, our findings indicate that azadirachtin negatively affects progeny output in adults’ females which survived from treated pupae compared to males. These findings provide clear evidence that azadirachtin causes negative physiological effects and decreases the offspring development rate in non-targeted species.
Azadirachtin, Drosophila melanogaster, Offspring development, Sublethal effects
