Dissipation and estimation of dietary risk of thiacloprid in pomegranate

Present study was undertaken to establish the dissipation trend of thiacloprid residues in pomegranate. Recovery studies were performed to validate the method's accuracy and quantified residues using an LCMS equipped with a C18 column. The initial deposit of thiacloprid 240 SC at 0.36 and 0.72 g. a.i. ha⁻¹ were 0.8 and 1.25 pg/g respectively. After 0, 1, 3, 5, 7, 10, 15, 20, 25, 30, 35 and 40 days from the last application, samples were collected. On 30 and 40 days after treatment (DAT), the thiacloprid residues were below the limit of quantification (BLQ = 0.05 μg/g) respective to the doses. The half-life period was 5.60 (X) and 9.52 (2X) days, with a safe waiting period of 13.09 and 14.33 days, respectively. Harvest time residues viz., mature fruit and soil samples were collected at 68 DAT. Juice and aril separated from mature fruit were subjected to residue analysis and showed no detectable thiacloprid residues.