Journal of Entomological Research

The present study intended to evaluate the joint larvicidal efficacy of Imidacloprid with the potent fraction of Ocimum basilicum and the impact of different environmental factors (light and temperature) for exploring suitable environmental conditions for preventing mosquito borne diseases. Investigation revealed that toxicity of the combination of O. basilicum potent fraction and imidacloprid was influenced by light and temperature conditions. Among the various light conditions, UV-1 h. and temperature, 30°C exhibited the maximum larvicidal toxicity against Anopheles stephensi. The UV-1 hour treated concentrations showed synergism with combined factor 3.6 and 4.5 for LC₅₀ and LC₉₀ values, respectively. Temperature 30°C with combined factor 2.0 and 1.61 for LC₅₀ and LC₉₀ values, subsequently shows synergism. Thus the photosensitization of the combination accelerates its bioactivity during UV light, whereas, the elevation of thermal gradient after 30°C increases the potency of the combination. The physical factors studied on the bioactivity of potent combination assist in the determination of time and mode of application of the synergist.