Validation and monitoring of chlorantraniliprole residues in paddy straw, paddy whole grain, unpolished rice and husk using LC-MS/MS

An efficient analytical method was developed and validated using a modified QuEChERS method and liquid chromatography mass spectroscopy/mass spectroscopy (LC-MS/MS) for the detection and quantification of chlorantraniliprole insecticide residues in paddy straw, paddy whole grain, unpolished rice, and husk samples. The method was validated by linearity, limits of detection, limit of quantification, precision and accuracy. The limits of detection was 0.004 μg/g while the limits of quantification were 0.012 μg/g, 0.013 μg/g, 0.012 μg/g and 0.011 μg/g, respectively for paddy straw, paddy whole grain, unpolished rice, and husk. Recovery study was done at five fortification levels ranging from 0.01 to 0.1 μg/mL. Recoveries of chlorantraniliprole ranged from 88.28 to 100.42, 85.17 to 94.22, 85.20 to 95.04, and 92.75 to 106.30%. Monitoring of farm gate samples revealed the presence of chlorantraniliprole residues (0.266, 0.07 and 0.05 mg/kg) in three samples out of 30 paddy straw samples tested. Also, out of 30 farm gate paddy whole grain samples tested, one sample was found contaminated with chlorantraniliprole residues (0.048 mg/kg).