1Department of Biotechnology and Microbiology, Noida International University, Greater Noida, Gautam Buddha Nagar - 201 310, Uttar Pradesh, India, E-mail: vc@niu.edu.in
2Department of Pharmacology, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth “Deemed to be University”, Karad, Satara - 415 539, Maharashtra, India, E-mail: pvpakale@gmail.com
3Department of Applied Sciences, CT University, Ludhiana - 142 024, Punjab, India, E-mail: tandonnitin12004@gmail.com
4School of Pharmaceutical Sciences, Lovely Professional University, Phagwara - 144 411, Punjab, India, E-mail: sorabh.lakhanpal@lpu.co.in
Krishna Institute of Science and Technology, Krishna Vishwa Vidyapeeth “Deemed to be University”, Karad, Satara - 415 539, Maharashtra, India
*Corresponding authors’ E-mail: sprajktaenvse@gmail.com
Online published on 18 February, 2026.
RNA interference (RNAi) technology is a breakthrough approach to eradicate certain pests in agriculture by using the RNA silences genes in natural ways. This research article delves further into how RNAi may be coupled with insect viruses to enhance approaches for pest management while also having less of an impact on the surroundings. It examines how RNAi functions, including molecular mechanisms and how microRNA (miRNA) and small interfering RNA (siRNA) target specific pest genes. Case examples demonstrating how RNAi has been effectively applied with insect viruses to manage main agricultural pests abound. Emphasising how stable, long-lasting, and practical they are in the field, the study report also examines other methods to deliver RNAi, including viral vectors and nanoparticles. Although using RNAi and insect susceptibility combined is a long-lasting and accurate approach to get rid of pests, there are still obstacles to tackle including political concerns, environmental stability, and the possibility of having unintended consequences.
Agriculture, Biocontrol, Biotechnology, Gene silencing, Insect pathogens, Pest suppression, Sustainable pest management, Transgenic
