1Department of Plant Pathology, C.S. Azad University of Agriculture & Technology, Kanpur 208 002, Uttar Pradesh, India;
2Division of Crop Protection, Indian Institute of Pulses Research, Kanpur 208 024, Uttar Pradesh, India;
*Present address: Division of Crop Protection, IIPR, Kanpur 208 024, Uttar Pradesh, India; Email: akram23859@gmail.com
A cowpea disease characterized by chlorotic and necrotic lesions in the leaves and necrosis on stem was noticed at Kanpur. The causal virus on sap inoculations from field infected cowpea to healthy cowpea cv. Pusa Komal produced chlorotic/necrotic lesions. Reverse transcriptase-PCR using primer pair RKJ5–5’ATG TCT CGC TTD TCT AAH GTB3’/RKJ6–5’TTA TAT TTC AAG AAG ATT ATC3’ for amplification of non structural movement (NSm) protein gene located on M (medium)-RNA of Groundnut bud necrosis virus (GBNV) was performed and an expected size ~900 bp DNA fragment was obtained indicating the involvement of GBNV. The partial sequence of NSm gene comprised of 819 nucleotides and a single ORF encoding for 273 amino acids. Analysis of partial NSm gene sequence revealed that the virus isolate causing necrosis disease of cowpea at Kanpur is an isolate of GBNV resembling closely with GBNV type isolate (99% homology at amino acid levels and 95% at nucleotide level) and has been designated as GBNV-CK (Accession number EU295662).
NSm gene, GBNV, Tospovirus, Cowpea
